The neuroblastoma SK-ER3 cell line obtained by stable transfection of the human SK-N-BE cell line is proposed as a model for the study of estrogen receptor activity in cells of neural origin, In the SK-ER3 cell line the estrogen receptor, once activated, initiates a differentiation program leading to growth arrest, morphological changes, and acquisition of the dopaminergic phenotype, In the absence of estrogens, this program can be triggered by IGF-I, which can activate the unliganded estrogen receptor via the ras-pathway, It is proposed that this model system might recapitulate the events occurring in vivo during the differentiation of the nervous system and that IGF-I may play an important role in the activation of estrogen receptor at the very early stage of brain development affecting the differentiation of a number of hypothalamic and extrahypothalamic brain regions. (C) 1997 Elsevier Science Inc.
SK-ER3 neuroblastoma cells as a model for the study of estrogen influence on neural cells / P. Agrati, M. Garnier, C. Patrone, G. Pollio, S. Santagati, E. Vegeto, A. Maggi - In: Brain Research Bullettin[s.l] : elsevier, 1997. - pp. 519-523 (( convegno Vth International Conference on Hormones, Brain, and Behavior (ICHBB) tenutosi a TURIN, ITALY nel AUG 25-30, 1996.
SK-ER3 neuroblastoma cells as a model for the study of estrogen influence on neural cells
E. VegetoPenultimo
;A. MaggiUltimo
1997
Abstract
The neuroblastoma SK-ER3 cell line obtained by stable transfection of the human SK-N-BE cell line is proposed as a model for the study of estrogen receptor activity in cells of neural origin, In the SK-ER3 cell line the estrogen receptor, once activated, initiates a differentiation program leading to growth arrest, morphological changes, and acquisition of the dopaminergic phenotype, In the absence of estrogens, this program can be triggered by IGF-I, which can activate the unliganded estrogen receptor via the ras-pathway, It is proposed that this model system might recapitulate the events occurring in vivo during the differentiation of the nervous system and that IGF-I may play an important role in the activation of estrogen receptor at the very early stage of brain development affecting the differentiation of a number of hypothalamic and extrahypothalamic brain regions. (C) 1997 Elsevier Science Inc.File | Dimensione | Formato | |
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