Clone CSA7 is a CHEF18 hamster cell line that shows an increased intracellular accumulation of dCTP. To localize the mutations that accumulate spontaneously in a functional gene of such a mutator phenotype, independent CSA7 mutants of the hypoxanthine-guanine phosphoribosyl transferase (hprt) gene were isolated and screened by a polymerase chain reaction-single strand conformation polymorphism technique. Sixty-two percent of mutants produced detectable changes of the strand migration profile and the mutations were preferentially localized in the exons 3 (31%) and 6 (62%). The sequencing of such exons revealed that the rate of C base incorporation was the major mutation pathway and that the A base of a GGA sequence was the preferential site of misincorporation.

dCTP misincorporation in a Chinese hamster mutator phenotype: the role of GGA genetic context / G. Rainaldi, R. Meneveri, L. Mariani, E. Ginelli, A. Moretti, L. Vatteroni. - In: MUTAGENESIS. - ISSN 0267-8357. - 11:4(1996 Jul), pp. 401-404.

dCTP misincorporation in a Chinese hamster mutator phenotype: the role of GGA genetic context

E. Ginelli;
1996

Abstract

Clone CSA7 is a CHEF18 hamster cell line that shows an increased intracellular accumulation of dCTP. To localize the mutations that accumulate spontaneously in a functional gene of such a mutator phenotype, independent CSA7 mutants of the hypoxanthine-guanine phosphoribosyl transferase (hprt) gene were isolated and screened by a polymerase chain reaction-single strand conformation polymorphism technique. Sixty-two percent of mutants produced detectable changes of the strand migration profile and the mutations were preferentially localized in the exons 3 (31%) and 6 (62%). The sequencing of such exons revealed that the rate of C base incorporation was the major mutation pathway and that the A base of a GGA sequence was the preferential site of misincorporation.
Animals; Cricetulus; Exons; Polymorphism, Single-Stranded Conformational; Hypoxanthine Phosphoribosyltransferase; Phenotype; Polymerase Chain Reaction; Base Sequence; DNA; Deoxycytosine Nucleotides; Molecular Sequence Data; Mutation; Cell Line; Cricetinae
Settore BIO/13 - Biologia Applicata
lug-1996
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/198200
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