A genotypic study using amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA fingerprinting (RAPD) and ribosomal spacer analysis (RSA) in comparison with DNA-DNA reassociation experiments was carried out with 85 thermophilic Bacillus isolates from uncultivated soil of 14 different geographical areas and seventeen reference strains representing defined thermophilic Bacillus species. This approach permitted the attribution of 51 % of the new isolates to the Bacillus thermoleovorans group and the identification of 40% of the new isolates as B. “thermodenitrificans”. Moreover, 2 strains were assigned to B. pallidus species and 1 isolate to B. thermosphaericus species. The remaining 6% of our thermophilic isolates from soil, constituting 2 DNA-DNA homology groups, are still unidentified. A detailed genotypic characterization of the heterogeneous species of B. thermoleovorans and B. stearothermophilus was also presented.

Genotypic characterization of thermophilic bacilli: a study on new soil isolates and several reference strains / D. Mora, M. Fortina, G. Nicastro, C. Parini, P. Manachini. - In: RESEARCH IN MICROBIOLOGY. - ISSN 0923-2508. - 149:10(1998), pp. 711-722.

Genotypic characterization of thermophilic bacilli: a study on new soil isolates and several reference strains

D. Mora
Primo
;
M. Fortina
Secondo
;
C. Parini
Penultimo
;
P. Manachini
Ultimo
1998

Abstract

A genotypic study using amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA fingerprinting (RAPD) and ribosomal spacer analysis (RSA) in comparison with DNA-DNA reassociation experiments was carried out with 85 thermophilic Bacillus isolates from uncultivated soil of 14 different geographical areas and seventeen reference strains representing defined thermophilic Bacillus species. This approach permitted the attribution of 51 % of the new isolates to the Bacillus thermoleovorans group and the identification of 40% of the new isolates as B. “thermodenitrificans”. Moreover, 2 strains were assigned to B. pallidus species and 1 isolate to B. thermosphaericus species. The remaining 6% of our thermophilic isolates from soil, constituting 2 DNA-DNA homology groups, are still unidentified. A detailed genotypic characterization of the heterogeneous species of B. thermoleovorans and B. stearothermophilus was also presented.
ARDRA-ITS; DNA-DNA homology; Identification; RAPD; RSA; Thermophilic Bacillus; ARDRA
Settore AGR/16 - Microbiologia Agraria
1998
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/196522
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 64
  • ???jsp.display-item.citation.isi??? 63
social impact