19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol have been suggested as possible adrenal scanning agents. This study compares these selenium-labelled cholesterol analogues and cholesterol in some of the metabolic transformations in which cholesterol is involved in mammalian systems. The activity of cholesterol 7a-hydroxylase, lecithin: cholesterol acyltransferase, microsomal cholesterol acyltransferase and the sterol side-chain cleavage enzyme systems to transform the mentioned cholesterol analogues has been investigated. The uptake and secretion in bile of 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol has been evaluated. The tissue distribution of these sterol analogues has been studied in rats. The findings suggest that 6-[75Se]-methyl-seleno-methyl-19-nor-cholest-5(10)en-3β-ol should be a useful agent for adrenal scanning.
In vitro and in vivo studies on two 75Se labelled cholesterol analogues: 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3 beta-ol / E. Bosisio, G. S. Boyd. - In: JOURNAL OF STEROID BIOCHEMISTRY. - ISSN 0022-4731. - 11:2(1979 Aug), pp. 1113-1119. [10.1016/0022-4731(79)90161-4]
In vitro and in vivo studies on two 75Se labelled cholesterol analogues: 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3 beta-ol
E. BosisioPrimo
;
1979
Abstract
19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol have been suggested as possible adrenal scanning agents. This study compares these selenium-labelled cholesterol analogues and cholesterol in some of the metabolic transformations in which cholesterol is involved in mammalian systems. The activity of cholesterol 7a-hydroxylase, lecithin: cholesterol acyltransferase, microsomal cholesterol acyltransferase and the sterol side-chain cleavage enzyme systems to transform the mentioned cholesterol analogues has been investigated. The uptake and secretion in bile of 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol has been evaluated. The tissue distribution of these sterol analogues has been studied in rats. The findings suggest that 6-[75Se]-methyl-seleno-methyl-19-nor-cholest-5(10)en-3β-ol should be a useful agent for adrenal scanning.
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