One of the transformation products formed from stearoyl CoA by the soluble enzymes of rat liver homogenate has been identified as 3 hydroxyoctadecanoic acid. The chirality of this acid obtained from incubation of [1 14C]stearoyl CoA with the 105,000 g soluble enzymes of rat liver has been determined. After 10 and 20 min of incubation 70% of the L(+) and 30% of the D(-) enantiomer are formed, whereas racemic 3 hydroxyoctadecanoic acid is isolated after 90 min of incubation. These results suggest either that an epimerizing enzyme is present in the soluble fraction or that 2 enzymes, each specifically forming one of the 2 enantiomers, are present.
Chirality of 3 hydroxyoctadecanoic acid from stearoyl CoA by rat liver soluble enzymes / M. Galli Kienle, G.M. Cighetti, E. Santaniello. - In: BIOORGANIC CHEMISTRY. - ISSN 0045-2068. - 4:1(1975), pp. 64-71. [10.1016/0045-2068(75)90026-7]
Chirality of 3 hydroxyoctadecanoic acid from stearoyl CoA by rat liver soluble enzymes
G.M. CighettiSecondo
;E. SantanielloUltimo
1975
Abstract
One of the transformation products formed from stearoyl CoA by the soluble enzymes of rat liver homogenate has been identified as 3 hydroxyoctadecanoic acid. The chirality of this acid obtained from incubation of [1 14C]stearoyl CoA with the 105,000 g soluble enzymes of rat liver has been determined. After 10 and 20 min of incubation 70% of the L(+) and 30% of the D(-) enantiomer are formed, whereas racemic 3 hydroxyoctadecanoic acid is isolated after 90 min of incubation. These results suggest either that an epimerizing enzyme is present in the soluble fraction or that 2 enzymes, each specifically forming one of the 2 enantiomers, are present.
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