We report the conditions (buffer composition and enzyme activity) required for estimating three frequently determined analytes--urea, glucose, and creatinine--by use of an improved version of the differential pH apparatus previously described (Clin Chem 29: 80-85, 1983). For each analyte, we used only one specific enzyme, thus avoiding a chain of auxiliary and indicator reactions. The method requires about a minute for each determination in undiluted plasma or whole blood.
Urea, creatinine, and glucose determined in plasma and whole blood by a differential pH technique / M. Ripamonti, A. Mosca, E. Rovida, M. Luzzana, L. Luzi, F. Ceriotti, F. Cottini, L. Rossi-Bernardi. - In: CLINICAL CHEMISTRY. - ISSN 0009-9147. - 30:4(1984 Apr), pp. 556-9-559.
Urea, creatinine, and glucose determined in plasma and whole blood by a differential pH technique
A. MoscaSecondo
;L. Luzi;
1984
Abstract
We report the conditions (buffer composition and enzyme activity) required for estimating three frequently determined analytes--urea, glucose, and creatinine--by use of an improved version of the differential pH apparatus previously described (Clin Chem 29: 80-85, 1983). For each analyte, we used only one specific enzyme, thus avoiding a chain of auxiliary and indicator reactions. The method requires about a minute for each determination in undiluted plasma or whole blood.
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