The effects of partial ileal bypass on plasma lipids, tissue cholesterol biosynthesis, and liver 7α-hydroxylation were studied in rabbits on a normal diet and after cholesterol feeding. The surgical procedure was minimally effective in control animals, whereas it dramatically reduced (even below baseline) plasma cholesterol levels in the diet-treated group. Liver cholesterol biosynthesis from [14C]acetate was not detectable in control rabbits. In these, biosynthesis from [14C]mevalonate was revealed in the liver and intestine, particularly in the duodenum. Cholesterol feeding totally suppressed the intestinal and liver biosynthetic activity, both from [14C]acetate and [14C]mevalonate. Partial ileal bypass significantly raised liver cholesterol biosynthesis from [14C]mevalonate, particularly in animals on the hyper-cholesterolemic regimen. No cholesterol 7α-hydroxylase activity, as determined by a mass fragmentographic technique, was found in control and cholesterol-fed rabbits; after partial ileal bypass, activities of 167 and 643 pmol·mg protein−1·hr−1, respectively, were found in controls and hypercholesterolemic animals. These findings underline the sensitivity of rabbits both to cholesterol feeding and to partial ileal bypass, a condition possibly related to the very low sterol synthetic activity in these animals. They also suggest that the surgical procedure may alter some regulatory step in tissue cholesterol biosynthesis

Cholesterol and bile-acid metabolism in normal and cholesterol-fed rabbits after partial lleal bypass / M. Lovati, M. Mora, E. Bosisio, G. Majone, M. Galli Kienle, G. Galli, C. Sirtori. - In: EXPERIMENTAL AND MOLECULAR PATHOLOGY. - ISSN 0014-4800. - 37:2(1982), pp. 156-165.

Cholesterol and bile-acid metabolism in normal and cholesterol-fed rabbits after partial lleal bypass

M. Lovati
Primo
;
E. Bosisio;C. Sirtori
Ultimo
1982

Abstract

The effects of partial ileal bypass on plasma lipids, tissue cholesterol biosynthesis, and liver 7α-hydroxylation were studied in rabbits on a normal diet and after cholesterol feeding. The surgical procedure was minimally effective in control animals, whereas it dramatically reduced (even below baseline) plasma cholesterol levels in the diet-treated group. Liver cholesterol biosynthesis from [14C]acetate was not detectable in control rabbits. In these, biosynthesis from [14C]mevalonate was revealed in the liver and intestine, particularly in the duodenum. Cholesterol feeding totally suppressed the intestinal and liver biosynthetic activity, both from [14C]acetate and [14C]mevalonate. Partial ileal bypass significantly raised liver cholesterol biosynthesis from [14C]mevalonate, particularly in animals on the hyper-cholesterolemic regimen. No cholesterol 7α-hydroxylase activity, as determined by a mass fragmentographic technique, was found in control and cholesterol-fed rabbits; after partial ileal bypass, activities of 167 and 643 pmol·mg protein−1·hr−1, respectively, were found in controls and hypercholesterolemic animals. These findings underline the sensitivity of rabbits both to cholesterol feeding and to partial ileal bypass, a condition possibly related to the very low sterol synthetic activity in these animals. They also suggest that the surgical procedure may alter some regulatory step in tissue cholesterol biosynthesis
Settore BIO/14 - Farmacologia
1982
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/191770
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