Serotonin (5HT) containing 180 instead of 160 was found in brain after exposing rats to an atmosphere of 1802. The 180 incorporation appeared linear with time and 22% of the 5HT in striatum contained 180 after one hour of exposure. 180 entered 5HT by enzymatic reaction rather than by isotope exchange as treatment with p-chlorophenylalanine, to block tryptophan hydroxylase, prevented incorporation, while more 180-5HT than normal was found in brain after treatment with pargyline, to block monoamine oxidase. Our studies suggest that the incorporation of 1802 into brain 5HT might be a feasible approach for evaluating 5HT turnover in animals and with some modification might be applied in man.
Incorporation of 18O2 into brain serotonin in vivo as a procedure for estimating turnover: a feasibility study in animals / C.L. Galli, J.W. Commissiong, E. Costa, N.H. Neff. - In: LIFE SCIENCES. - ISSN 0024-3205. - 22:6(1978 Feb), pp. 473-477.
Incorporation of 18O2 into brain serotonin in vivo as a procedure for estimating turnover: a feasibility study in animals
C.L. GalliPrimo
;
1978
Abstract
Serotonin (5HT) containing 180 instead of 160 was found in brain after exposing rats to an atmosphere of 1802. The 180 incorporation appeared linear with time and 22% of the 5HT in striatum contained 180 after one hour of exposure. 180 entered 5HT by enzymatic reaction rather than by isotope exchange as treatment with p-chlorophenylalanine, to block tryptophan hydroxylase, prevented incorporation, while more 180-5HT than normal was found in brain after treatment with pargyline, to block monoamine oxidase. Our studies suggest that the incorporation of 1802 into brain 5HT might be a feasible approach for evaluating 5HT turnover in animals and with some modification might be applied in man.
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