A preparation method of calcium alginate beads containing swine spermatozoa was developed. A suspension usually employed for artificial insemination and containing living spermatozoa, to which was added hydroxypropylmethylcellulose and calcium chloride, was dropped into a sodium alginate solution. Calcium ions diffusing out of the droplets, reacted with the sodium alginate, leading to the formation of a water-insoluble calcium alginate gel membrane. Half of the capsules obtained was cross-linked by interfacial polymerization using an aqueous solution of protamine sulphate. The two kinds of capsules (cross-linked and not) containing spermatozoa were then transferred to a suitable extender for swine sperm and their morphology (scanning electron microscope) and in vitro sperm viability (survival time, motility and acrosomal integrity) was studied.
Calcium alginate capsules containing a hydrophilic polymer for the encapsulation of swine spermatozoa / M.L. Torre, L. Maggi, P. Giunchedi, U. Conte, D. Vigo, G. Maffeo. - In: STP PHARMA SCIENCES. - ISSN 1157-1489. - 8:4(1998), pp. 233-236.
Calcium alginate capsules containing a hydrophilic polymer for the encapsulation of swine spermatozoa
D. Vigo;G. Maffeo
1998
Abstract
A preparation method of calcium alginate beads containing swine spermatozoa was developed. A suspension usually employed for artificial insemination and containing living spermatozoa, to which was added hydroxypropylmethylcellulose and calcium chloride, was dropped into a sodium alginate solution. Calcium ions diffusing out of the droplets, reacted with the sodium alginate, leading to the formation of a water-insoluble calcium alginate gel membrane. Half of the capsules obtained was cross-linked by interfacial polymerization using an aqueous solution of protamine sulphate. The two kinds of capsules (cross-linked and not) containing spermatozoa were then transferred to a suitable extender for swine sperm and their morphology (scanning electron microscope) and in vitro sperm viability (survival time, motility and acrosomal integrity) was studied.Pubblicazioni consigliate
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