Capillary gas chromatographic determination of spermidine in diet integrators

Biogenic polyamines, among which is spermidine (SPD, NH2-(CH2)4-NH-(CH2)3-NH2), are ubiquitous polycationic mols. that have a definitive role in many biol. processes, such as nucleic acid metab., protein synthesis, and cell growth. SPD is present in diet integrators because it seems to favor the hair growth. This work describes a capillary gas chromatog. (CGC) method for the quant. detn. of SPD in diet integrators using cadaverine internal std. (IS), a Me siliconic capillary column and flame-ionization detector (FID). Diet tablets, contg. SPD, are pulverized; an aliquot of powder is treated with an alk. aq. soln. and added with IS. The suspension is extd. with di-Et ether contg. Et chloroformate (ECF). The ether exts., evapd. to dryness and reconstituted in Et acetate were analyzed in CGC/FID. Derivs. of polyamines with ECF were characterized in CGC/MS too. Validation has considered specificity, linearity, precision and accuracy of anal. method; this parameters are valid for the quant. detn. of SPD in diet integrators