In 1992, DNA amplification in polymerase chain reaction (PCR) mixtures containing template DNA extracted from naturally infected grapevine was used to identify a mycoplasma-like organisms (MLO) in plants showing flavescence doree (FD) symptoms in the Lombardia region of northern Italy. Oligonucleotide pair rl6SF2/rl6SR2 primed amplification of an MLO-characteristic 1200 bp fragment of 16S ribosomal DNA. Digestion of the fragment (using Alu I, Mse I or Kpn I) yielded RFLP patterns similar to those characteristic of 16S rDNA from aster yellows MLO strain cluster (16S rRNA gene group I). The MLO in grapevine was further identified by PCR using other oligonucleotide primers previously designed for MLO group-specific amplification of DNA and by restriction analysis of the amplified DNA. The Lombardian MLO was identical to 2 aster yellows-related MLO strains isolated from diseased grapevine in the Emilia-Romagna region, but was distinct from an FD-associated MLO from the Friuli-Venezia Giulia region. In experiments conducted in 1993, the presence of aster yellows MLO in grapevine was confirmed, and some vines in northern Italy were doubly infected by aster yellows and elm yellows MLOs. It is concluded that genetically diverse MLOs are associated with grapevine yellows diseases in northern Italy.

Double and single infections by aster yellows and elm yellows MLOs in grapevines with symptoms characteristic of flavescence doree / P.A. Bianco, R.E. Davis, J.P. Prince, I.M. Lee, D.E. Gundersen, A. Fortusini, G. Belli. - In: RIVISTA DI PATOLOGIA VEGETALE. - ISSN 0035-6441. - 3:3(1993).

Double and single infections by aster yellows and elm yellows MLOs in grapevines with symptoms characteristic of flavescence doree

P.A. Bianco
Primo
;
A. Fortusini
Penultimo
;
G. Belli
Ultimo
1993

Abstract

In 1992, DNA amplification in polymerase chain reaction (PCR) mixtures containing template DNA extracted from naturally infected grapevine was used to identify a mycoplasma-like organisms (MLO) in plants showing flavescence doree (FD) symptoms in the Lombardia region of northern Italy. Oligonucleotide pair rl6SF2/rl6SR2 primed amplification of an MLO-characteristic 1200 bp fragment of 16S ribosomal DNA. Digestion of the fragment (using Alu I, Mse I or Kpn I) yielded RFLP patterns similar to those characteristic of 16S rDNA from aster yellows MLO strain cluster (16S rRNA gene group I). The MLO in grapevine was further identified by PCR using other oligonucleotide primers previously designed for MLO group-specific amplification of DNA and by restriction analysis of the amplified DNA. The Lombardian MLO was identical to 2 aster yellows-related MLO strains isolated from diseased grapevine in the Emilia-Romagna region, but was distinct from an FD-associated MLO from the Friuli-Venezia Giulia region. In experiments conducted in 1993, the presence of aster yellows MLO in grapevine was confirmed, and some vines in northern Italy were doubly infected by aster yellows and elm yellows MLOs. It is concluded that genetically diverse MLOs are associated with grapevine yellows diseases in northern Italy.
DNA ; fruit crops ; grapes ; identification ; molecular genetics ; mycoplasma-like organisms ; plant diseases ; plant pathogens ; plant pathology ; plant viruses ; techniques
Settore AGR/12 - Patologia Vegetale
1993
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/179424
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