Cyanobacterial response to three biocide strategies: (i) a commercial isothiazoline biocide (Biotin T®), (ii) high temperature (37ºC) and (iii) UV-C germicidal irradiation on short term was studied with the aim of assessing the usefulness of CIELAB color parameters (L*, a*, b*, C*ab and hºab) in monitoring the effectiveness of biocide treatments. The experimental setup consisted of several samples from the same culture, a pure planktonic culture of an aeroterrestrial stone biofilm-forming cyanobacterium of the genus Nostoc (PCC 9104), testing the three biocide strategies at the same time. CIELAB color data were supported by spectrophotometric measurements of chlorophyll a, phycobiliproteins and total carotenoids, as well as adenosine triphosphate (ATP) assay. Results suggested that some of the CIELAB parameters could be profitably used to monitor the effectiveness of biocide treatments.
Evaluation of three biocide strategies in planktonic Nostoc sp. PCC 9104 stone biofilm-forming cyanobacterium by quantification of the color variations / P. Sanmartín, F. Villa, B. Silva, F. Cappitelli, B. Prieto. ((Intervento presentato al 10. convegno International Conference on non-destructive investigations and microanalysis for the diagnostics and conservation of cultural and environmental heritage tenutosi a Firenze, Italy nel 2011.
Evaluation of three biocide strategies in planktonic Nostoc sp. PCC 9104 stone biofilm-forming cyanobacterium by quantification of the color variations
F. VillaSecondo
;F. CappitelliPenultimo
;
2011
Abstract
Cyanobacterial response to three biocide strategies: (i) a commercial isothiazoline biocide (Biotin T®), (ii) high temperature (37ºC) and (iii) UV-C germicidal irradiation on short term was studied with the aim of assessing the usefulness of CIELAB color parameters (L*, a*, b*, C*ab and hºab) in monitoring the effectiveness of biocide treatments. The experimental setup consisted of several samples from the same culture, a pure planktonic culture of an aeroterrestrial stone biofilm-forming cyanobacterium of the genus Nostoc (PCC 9104), testing the three biocide strategies at the same time. CIELAB color data were supported by spectrophotometric measurements of chlorophyll a, phycobiliproteins and total carotenoids, as well as adenosine triphosphate (ATP) assay. Results suggested that some of the CIELAB parameters could be profitably used to monitor the effectiveness of biocide treatments.
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