In dairy cattle breeding, herd reproductive management is the main topics and affects a large part of general costs. Bull infertility represents a significant component on unsuccessful pregnancy. In this study, proteomic analysis was applied to compare the sperm protein expression profile from bulls with a different level of fertility. Three commercial extenders were used in this study. Image analysis allowed the differentiation of the three extenders used for freezing. Comparative proteomic analysis shown that expression of several (5 different 2-D spots) proteins is related to fertility (p<0.05). Their physiological functions or biochemical properties sustain role in sperm functionality defects can compromise sperm chances to reach and fertilize the oocyte.
Proteomica applicata alla valutazione della fertilità del seme bovino congelato con differenti mestrui diluitori / P. Roncada, H. Ahmed Hussein Ahmed, C. Piras, A. Soggiu, A. Galli, E. Maroni, L. Bonizzi, A. Gaviraghi. - In: ATTI DELLA SOCIETÀ ITALIANA DELLE SCIENZE VETERINARIE. - ISSN 1825-4454. - Disco ottico. - 65:(2011), pp. 18-21. ((Pentium 3 - 1200 Mhz (consigliato 1700 MHZ), 256 Mb RAM (consigliato 512), Lettore CD-ROM 12x (consigliato 52x), Internet Explorer 6. - Intervento presentato al 65. convegno Convegno nazionale SISVet tenutosi a Tropea-Drapia (Vibo Valentia) nel 2011.
Proteomica applicata alla valutazione della fertilità del seme bovino congelato con differenti mestrui diluitori
P. Roncada;H. Ahmed Hussein Ahmed;C. Piras;A. Soggiu;L. Bonizzi;A. Gaviraghi
2011
Abstract
In dairy cattle breeding, herd reproductive management is the main topics and affects a large part of general costs. Bull infertility represents a significant component on unsuccessful pregnancy. In this study, proteomic analysis was applied to compare the sperm protein expression profile from bulls with a different level of fertility. Three commercial extenders were used in this study. Image analysis allowed the differentiation of the three extenders used for freezing. Comparative proteomic analysis shown that expression of several (5 different 2-D spots) proteins is related to fertility (p<0.05). Their physiological functions or biochemical properties sustain role in sperm functionality defects can compromise sperm chances to reach and fertilize the oocyte.
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