The measurement of glutathione is a challenging task in that, during sample manipulation, a large percentage of this compound can be artificially oxidized. Here a high-performance liquid chromatography (HPLC) method to measure reduced glutathione in blood, based on the analysis of its conjugate with N-ethylmaleimide, has been developed and validated. The method is linear in the concentration range 0.1-2 mM, and the lower limit of quantification is 0.05 mM. Blood samples treated with N-ethylmaleimide are stable at -20 degrees C for 90 days. This method has simplicity and rapidity as its main advantages; therefore, it is particularly suitable for large-scale clinical studies.
Detection of glutathione in whole blood after stabilization with N-ethylmaleimide / D. Giustarini, I. Dalle Donne, A. Milzani, R. Rossi. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - 415:1(2011), pp. 81-83. [10.1016/j.ab.2011.04.013]
Detection of glutathione in whole blood after stabilization with N-ethylmaleimide
I. Dalle Donne;A. Milzani;
2011
Abstract
The measurement of glutathione is a challenging task in that, during sample manipulation, a large percentage of this compound can be artificially oxidized. Here a high-performance liquid chromatography (HPLC) method to measure reduced glutathione in blood, based on the analysis of its conjugate with N-ethylmaleimide, has been developed and validated. The method is linear in the concentration range 0.1-2 mM, and the lower limit of quantification is 0.05 mM. Blood samples treated with N-ethylmaleimide are stable at -20 degrees C for 90 days. This method has simplicity and rapidity as its main advantages; therefore, it is particularly suitable for large-scale clinical studies.
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