The major goal of our research is the development of synthetically useful selective biotransformation using “new” microbial enzymes by combining natural and non-natural condition. The production of optically pure molecules to be used in different fields has been studied. A different new chemo-, regio- and enantio-selective carboxylesterases are selected after screening among different microorganisms. Medium and biocatalyst manipulation and reactor engineering are studied for improving the selectivity/productivity of the bioprocesses. The resolution of racemic esters by hydrolysis (i.e. isopropylideneglycerol or non-natural aminoacid) or esters synthesis (i.e. flurbiprofene) with lyophilised cells is obtained. (R) and (S)-IPG are obtained using two different biocatalysts: an esterase from Bacillus coagulans, as immobilized or isolated enzyme, and one from Kluyveromyces marxianus.
Production of optically pure molecules with new microbial enzyme / D. Romano, V. Ferrario, N.A. Ferrara, R. Gualandris, F.E. Molinari, R. Gandolfi. ((Intervento presentato al 8. convegno Convegno FISV tenutosi a Riva del Garda nel 2006.
Production of optically pure molecules with new microbial enzyme
D. Romano;N.A. Ferrara;R. Gualandris;F.E. Molinari;R. Gandolfi
2006
Abstract
The major goal of our research is the development of synthetically useful selective biotransformation using “new” microbial enzymes by combining natural and non-natural condition. The production of optically pure molecules to be used in different fields has been studied. A different new chemo-, regio- and enantio-selective carboxylesterases are selected after screening among different microorganisms. Medium and biocatalyst manipulation and reactor engineering are studied for improving the selectivity/productivity of the bioprocesses. The resolution of racemic esters by hydrolysis (i.e. isopropylideneglycerol or non-natural aminoacid) or esters synthesis (i.e. flurbiprofene) with lyophilised cells is obtained. (R) and (S)-IPG are obtained using two different biocatalysts: an esterase from Bacillus coagulans, as immobilized or isolated enzyme, and one from Kluyveromyces marxianus.Pubblicazioni consigliate
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