Background:We conducted the first analysis of viral microRNAs (miRNAs) in lung cancer, with a focus on Epstein-Barr virus (EBV).Methods:We evaluated viral miRs with a two-channel oligo-array targeting mature, anti-sense miRNAs in 290 cases. In 48 cases, we compared microarray and real-time quantitative PCR (qPCR) expression for three EBV miRNAs. We tested for EBV DNA, RNA, and protein in tumour tissue from six cases with and six cases without strong qPCR-based evidence of EBV miRNAs.Results:The EBV miRNAs strongly differentiated between adenocarcinoma and squamous cell carcinoma using the microarray (P=0.01 for 9 out of 16 EBV miRNAs). However, microarray and qPCR measurements of BART1, BART2, and BHRF1-3 expression were not significantly correlated (P=0.53, 0.94, and 0.47, respectively). Although qPCR provided substantial evidence of EBV miRNAs in 7 out of 48 cases, only 1 of these 7 cases had detectable EBV DNA in tumour tissue. None had detectable EBV RNA or protein by histochemical stains.Conclusion:In a comprehensive evaluation of EBV miRNA, DNA, RNA, and protein in lung cancer, we found little evidence of EBV in lung tumour tissue. Discrepancies between microarray-and qPCR-based strategies highlight the difficulty of validating molecular markers of disease. Our results do not support a role of EBV in lung cancer.

Epstein-barr virus virus microRNA and lung cancer / J. Koshiol, M.L. Gulley, Y. Zhao, M. Rubagotti, F.M. Marincola, M. Rotunno, W. Tang, A.W. Bergen, P.A. Bertazzi, D. Roy, A.C. Pesatori, I. Linnoila, D. Dittmer, A.M. Goldstein, N.E. Caporaso, L.M. Mcshane, E. Wang, M.T. Landi. - In: BRITISH JOURNAL OF CANCER. - ISSN 0007-0920. - 105:2(2011 Jul 12), pp. 320-326. [10.1038/bjc.2011.221]

Epstein-barr virus virus microRNA and lung cancer

M. Rubagotti;P.A. Bertazzi;A.C. Pesatori;
2011

Abstract

Background:We conducted the first analysis of viral microRNAs (miRNAs) in lung cancer, with a focus on Epstein-Barr virus (EBV).Methods:We evaluated viral miRs with a two-channel oligo-array targeting mature, anti-sense miRNAs in 290 cases. In 48 cases, we compared microarray and real-time quantitative PCR (qPCR) expression for three EBV miRNAs. We tested for EBV DNA, RNA, and protein in tumour tissue from six cases with and six cases without strong qPCR-based evidence of EBV miRNAs.Results:The EBV miRNAs strongly differentiated between adenocarcinoma and squamous cell carcinoma using the microarray (P=0.01 for 9 out of 16 EBV miRNAs). However, microarray and qPCR measurements of BART1, BART2, and BHRF1-3 expression were not significantly correlated (P=0.53, 0.94, and 0.47, respectively). Although qPCR provided substantial evidence of EBV miRNAs in 7 out of 48 cases, only 1 of these 7 cases had detectable EBV DNA in tumour tissue. None had detectable EBV RNA or protein by histochemical stains.Conclusion:In a comprehensive evaluation of EBV miRNA, DNA, RNA, and protein in lung cancer, we found little evidence of EBV in lung tumour tissue. Discrepancies between microarray-and qPCR-based strategies highlight the difficulty of validating molecular markers of disease. Our results do not support a role of EBV in lung cancer.
Epstein-Barr virus; lung cancer; microarray; microRNA; qPCR
Settore MED/44 - Medicina del Lavoro
12-lug-2011
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/164080
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