Induced resistance by plant activators represents a promising perspective in crop protection (Gozzo, 2004). As elicitors, these products are able to boost the plant own defences against a broad spectrum of pathogens, a phenomenon known as systemic acquired resistance (SAR). Among plant activators, benzothiadiazole (BTH) and chitosan proved to be effective in controlling grapevine fungal disease (Iriti et al., 2004; Dagostin et al., 2006; Trotel-Aziz et al., 2006), meanwhile improving the content of grape antioxidant compounds, i.e. anthocyanis, proanthocyanidins, stilbenes and melatonin (Iriti et al., 2004, 2005, 2006, 2008). In this work, we assayed the total polyphenol (TP) content and antioxidant power of two different grapevine (Vitis vinifera L.) cultivars, Montepulciano d’Abruzzo and Merlot, treated with kendal-COPS® (kC® Valagro, a formulation based on chitosan and Cu and Mn as microelements) and with phosphite (potassium phosphonate), respectively. Furthermore, we determined the TP content and antioxidant power of the experimental wines produced by microvinification of Montepulciano d’Abruzzo treated and untreated grapes. Our results showed that the TP content, measured by Folin-Ciocalteau colorimetric assay, was higher in all kC®- and phosphite-treated grape berry tissues of both cultivars, compared with either untreated controls or conventional fungicide treated plants. A similar trend was observed with regard to the antioxidant power of the grapes, assessed by DPPH (2,2-diphenyl-1-pycryl hydrazyl) free radical scavenging test. However, the results obtained from the analysis of Montepulciano d’Abruzzo wine revealed a different pattern, with a higher TP content and antioxidant power in the sample produced from control grapes in respect to those obtained from both kC®- and fungicide-treated grapes. This discrepancy could be due to the skin tissue thickening induced by kC®, a typical plant defence response, which could reduce the polyphenol extraction during microvinification, but would hardly occur in a conventional winemaking process. Though this aspect needs further investigation, it can be concluded that open field treatments with plant resistance activators deserve particular attention, not only as a low-environmental impact strategy in the management of grapevine fungal diseases, but also because they contribute to improve the bioactive phytochemicals content of grape.

Effects of chitosan and phosphite treatments on total polyphenol content and antioxidant power of different grape berry tissues and wines / S. Vitalini, F. Faoro, G. Di Tommaso, D. Di Tommaso, M. Borgo, A. Piaggesi, G. Fico, F. Tomé, M. Iriti. ((Intervento presentato al convegno Resistenza indotta per il controllo di malattie delle piante : efficacia e meccanismi di azione di uno strumento sostenibile tenutosi a Ancona nel 2009.

Effects of chitosan and phosphite treatments on total polyphenol content and antioxidant power of different grape berry tissues and wines

S. Vitalini
Primo
;
F. Faoro
Secondo
;
G. Fico;F. Tomé
Penultimo
;
M. Iriti
Ultimo
2009

Abstract

Induced resistance by plant activators represents a promising perspective in crop protection (Gozzo, 2004). As elicitors, these products are able to boost the plant own defences against a broad spectrum of pathogens, a phenomenon known as systemic acquired resistance (SAR). Among plant activators, benzothiadiazole (BTH) and chitosan proved to be effective in controlling grapevine fungal disease (Iriti et al., 2004; Dagostin et al., 2006; Trotel-Aziz et al., 2006), meanwhile improving the content of grape antioxidant compounds, i.e. anthocyanis, proanthocyanidins, stilbenes and melatonin (Iriti et al., 2004, 2005, 2006, 2008). In this work, we assayed the total polyphenol (TP) content and antioxidant power of two different grapevine (Vitis vinifera L.) cultivars, Montepulciano d’Abruzzo and Merlot, treated with kendal-COPS® (kC® Valagro, a formulation based on chitosan and Cu and Mn as microelements) and with phosphite (potassium phosphonate), respectively. Furthermore, we determined the TP content and antioxidant power of the experimental wines produced by microvinification of Montepulciano d’Abruzzo treated and untreated grapes. Our results showed that the TP content, measured by Folin-Ciocalteau colorimetric assay, was higher in all kC®- and phosphite-treated grape berry tissues of both cultivars, compared with either untreated controls or conventional fungicide treated plants. A similar trend was observed with regard to the antioxidant power of the grapes, assessed by DPPH (2,2-diphenyl-1-pycryl hydrazyl) free radical scavenging test. However, the results obtained from the analysis of Montepulciano d’Abruzzo wine revealed a different pattern, with a higher TP content and antioxidant power in the sample produced from control grapes in respect to those obtained from both kC®- and fungicide-treated grapes. This discrepancy could be due to the skin tissue thickening induced by kC®, a typical plant defence response, which could reduce the polyphenol extraction during microvinification, but would hardly occur in a conventional winemaking process. Though this aspect needs further investigation, it can be concluded that open field treatments with plant resistance activators deserve particular attention, not only as a low-environmental impact strategy in the management of grapevine fungal diseases, but also because they contribute to improve the bioactive phytochemicals content of grape.
giu-2009
fungal diseases ; grapevine phytoalexins ; induced resistance ; phytochemicals
Settore AGR/12 - Patologia Vegetale
Dipartimento di Scienze Ambientali e delle Produzioni Vegetali, Università Politecnica delle Marche
Effects of chitosan and phosphite treatments on total polyphenol content and antioxidant power of different grape berry tissues and wines / S. Vitalini, F. Faoro, G. Di Tommaso, D. Di Tommaso, M. Borgo, A. Piaggesi, G. Fico, F. Tomé, M. Iriti. ((Intervento presentato al convegno Resistenza indotta per il controllo di malattie delle piante : efficacia e meccanismi di azione di uno strumento sostenibile tenutosi a Ancona nel 2009.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/153972
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