A subcutaneous mass on the left antebrachium of an 11-year-old intact female English Pointer dog was evaluated presurgically by cytologic examination and immunocytochemical staining. The sample consisted of discrete, variably sized, markedly pleomorphic neoplastic cells that expressed vimentin with diffuse cytoplasmic staining, desmin with focal paranuclear staining, and myoglobin with diffuse cytoplasmic staining, consistent with a diagnosis of rhabdomyosarcoma. Lymphocytic and histiocytic markers were negative. Aspirates of the enlarged ipsilateral prescapular lymph node were positive for metastatic disease. Surgical excision of the tumor and lymph node were followed by histologic and electron microscopic examination. Histomorphologic appearance of neoplastic cells from the mass and the lymph node paralleled cytologic findings; the histologic diagnosis was round cell variant of embryonal rhabdomyosarcoma. By ultrastructural evaluation, cells contained numerous mitochondria and masses of cytoplasmic tangled myofilaments, features typical of rhabdomyoblasts. The dog received doxorubicin (30 mg/m(2) ) every 3 weeks for 5 treatments. Local recurrence developed 6 months after resection but was not treated. Despite a guarded prognosis and untreated local recurrence, the dog was still alive 18 months after surgery. Cytologic evaluation and immunocytochemical staining were pivotal for the presurgical diagnosis of rhabdomyosarcoma.

Subcutaneous embryonal rhabdomyosarcoma in a dog : cytologic, immunocytochemical, histologic, and ultrastructural features / G. Avallone, N. Pinto da Cunha, C. Palmieri, L. Della Salda, D. Stefanello, P. Roccabianca, M. Caniatti. - In: VETERINARY CLINICAL PATHOLOGY. - ISSN 0275-6382. - 39:4(2010 Dec), pp. 499-504. [10.1111/j.1939-165X.2010.00271.x]

Subcutaneous embryonal rhabdomyosarcoma in a dog : cytologic, immunocytochemical, histologic, and ultrastructural features

G. Avallone
Primo
;
N. Pinto da Cunha
Secondo
;
D. Stefanello;P. Roccabianca
Penultimo
;
M. Caniatti
Ultimo
2010

Abstract

A subcutaneous mass on the left antebrachium of an 11-year-old intact female English Pointer dog was evaluated presurgically by cytologic examination and immunocytochemical staining. The sample consisted of discrete, variably sized, markedly pleomorphic neoplastic cells that expressed vimentin with diffuse cytoplasmic staining, desmin with focal paranuclear staining, and myoglobin with diffuse cytoplasmic staining, consistent with a diagnosis of rhabdomyosarcoma. Lymphocytic and histiocytic markers were negative. Aspirates of the enlarged ipsilateral prescapular lymph node were positive for metastatic disease. Surgical excision of the tumor and lymph node were followed by histologic and electron microscopic examination. Histomorphologic appearance of neoplastic cells from the mass and the lymph node paralleled cytologic findings; the histologic diagnosis was round cell variant of embryonal rhabdomyosarcoma. By ultrastructural evaluation, cells contained numerous mitochondria and masses of cytoplasmic tangled myofilaments, features typical of rhabdomyoblasts. The dog received doxorubicin (30 mg/m(2) ) every 3 weeks for 5 treatments. Local recurrence developed 6 months after resection but was not treated. Despite a guarded prognosis and untreated local recurrence, the dog was still alive 18 months after surgery. Cytologic evaluation and immunocytochemical staining were pivotal for the presurgical diagnosis of rhabdomyosarcoma.
Desmin ; electron microscopy ; immunocytochemistry ; myoglobin ; skeletal muscle ; vimentin
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
Settore VET/09 - Clinica Chirurgica Veterinaria
dic-2010
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/150816
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