Introduction: Reversine, a synthetic purine, known to increase plasticity of terminally differentiated cells, has been studied in human adipose-derived stem cells (hASCs) Its effects on undifferentiated and differentiated cells have been evaluated. Materials and Methods: hASCs were treated for 72 hours with several concentrations of Reversine before differentiation towards osteogenic and myogenic lineages Cellular growth and viability were monitored, and the effect of Reversine on osteogenic and myogenic differentiation was also analyzed. Results: Reversine induces an increase of gene expression of alkaline phosphatase (ALP), compared to vehicle, (p< 01) and osteogenic markers such as ALP enzymatic activity and calcium deposition. In particular, we observed that 50nM dose produces a significant increase in ALP activity respect to vehicle (p< 01), and that this concentration does not show any affect on cell growth and viability, whereas concentrations 100 fold higher cause cell cytotoxicity. Moreover, Reversine seems to improve hASCs myogenic differentiation since we detected an induction of α-actin gene expression (p< 05) when hASCs switch into smooth muscle like cells, whereas, only after Reversine treatment, we observed human-nuclei localized into myotubes in skeletal muscle like cells generated after co-cultured of hASCs with murine C2C12 cells. Discussion: Reversine treatment seems to promote the differentiation process of hASCs into cells of the osteogenic and myogenic lineage.

Effects of reversine on human adipose stem cells plasticity / E. Arrigoni, D. Stanco, E. Conforti, S. Lopa, S. Niada, L. De Girolamo, L. Anastasia, A.T. Brini. ((Intervento presentato al 80. convegno Annual Conference of the International Federation of Adipose Therapeutics and Science (IFATS) tenutosi a Dallas nel 2010.

Effects of reversine on human adipose stem cells plasticity

E. Arrigoni;D. Stanco;S. Niada;L. De Girolamo;L. Anastasia;A.T. Brini
2010

Abstract

Introduction: Reversine, a synthetic purine, known to increase plasticity of terminally differentiated cells, has been studied in human adipose-derived stem cells (hASCs) Its effects on undifferentiated and differentiated cells have been evaluated. Materials and Methods: hASCs were treated for 72 hours with several concentrations of Reversine before differentiation towards osteogenic and myogenic lineages Cellular growth and viability were monitored, and the effect of Reversine on osteogenic and myogenic differentiation was also analyzed. Results: Reversine induces an increase of gene expression of alkaline phosphatase (ALP), compared to vehicle, (p< 01) and osteogenic markers such as ALP enzymatic activity and calcium deposition. In particular, we observed that 50nM dose produces a significant increase in ALP activity respect to vehicle (p< 01), and that this concentration does not show any affect on cell growth and viability, whereas concentrations 100 fold higher cause cell cytotoxicity. Moreover, Reversine seems to improve hASCs myogenic differentiation since we detected an induction of α-actin gene expression (p< 05) when hASCs switch into smooth muscle like cells, whereas, only after Reversine treatment, we observed human-nuclei localized into myotubes in skeletal muscle like cells generated after co-cultured of hASCs with murine C2C12 cells. Discussion: Reversine treatment seems to promote the differentiation process of hASCs into cells of the osteogenic and myogenic lineage.
ott-2010
Settore BIO/14 - Farmacologia
Settore BIO/10 - Biochimica
International Federation of Adipose Therapeutics and Science (IFATS)
http://ifats.org/wp-content/uploads/2010/10/IFATS-Program-Book.pdf
Effects of reversine on human adipose stem cells plasticity / E. Arrigoni, D. Stanco, E. Conforti, S. Lopa, S. Niada, L. De Girolamo, L. Anastasia, A.T. Brini. ((Intervento presentato al 80. convegno Annual Conference of the International Federation of Adipose Therapeutics and Science (IFATS) tenutosi a Dallas nel 2010.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/148792
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