From the beginning of October 2006 to January 31st 2008, in the context of a work sponsored by Merial Italia, a diagnostic pathway has been developed for the study of post-weaning multisystemic wasting syndrome (PMWS) of pigs. Superficial inguinal and bronchial lymph nodes, ileum, tonsil and lung from 3 to 5 subjects (229 pigs belonging to 64 farms from North and Centre Italy) were collected. The samples were valued by histological and immunohistochemical (IHC) assays for porcine circovirus-2 (PCV2). The diagnosis confirmed PMWS in those cases with inclusion bodies or where the presence of a positive IHC stain was contextual to the lesions indicative of PMWS in the lymphoid tissue (depletion and/or granulomas) and/or lung (interstitial pneumonia). In the other cases the samples were considered PCV2 infected when PCV2-positive cells were present without PMWS lesions; negative in the absence of PCV2 infection and PMWS lesions. PMWS was diagnosed on 48 farms; 21 where the total number of examined subjects had a conclusive diagnosis of PMWS, whereas the diagnosis could be considered conclusive for PMWS only for some animals on the remaining 27. The protocol proved more reliable when appled to a group of animals than to a single pig.

Diagnosi di PMWS in Italia / G. Sarli, F. Ostanello, F. Morandi, L. Fusaro, B. Bacci, A. Nigrielli, L. Alborali, M. Dottori, F. Vezzoli, G. Barigazzi, L. Fiorentini, V. Sala, G. Leotti. - In: LARGE ANIMALS REVIEW. - ISSN 1124-4593. - 14:3(2008), pp. 119-126.

Diagnosi di PMWS in Italia

V. Sala
Penultimo
;
2008

Abstract

From the beginning of October 2006 to January 31st 2008, in the context of a work sponsored by Merial Italia, a diagnostic pathway has been developed for the study of post-weaning multisystemic wasting syndrome (PMWS) of pigs. Superficial inguinal and bronchial lymph nodes, ileum, tonsil and lung from 3 to 5 subjects (229 pigs belonging to 64 farms from North and Centre Italy) were collected. The samples were valued by histological and immunohistochemical (IHC) assays for porcine circovirus-2 (PCV2). The diagnosis confirmed PMWS in those cases with inclusion bodies or where the presence of a positive IHC stain was contextual to the lesions indicative of PMWS in the lymphoid tissue (depletion and/or granulomas) and/or lung (interstitial pneumonia). In the other cases the samples were considered PCV2 infected when PCV2-positive cells were present without PMWS lesions; negative in the absence of PCV2 infection and PMWS lesions. PMWS was diagnosed on 48 farms; 21 where the total number of examined subjects had a conclusive diagnosis of PMWS, whereas the diagnosis could be considered conclusive for PMWS only for some animals on the remaining 27. The protocol proved more reliable when appled to a group of animals than to a single pig.
Diagnosis; Histopathology.; Immunohistochemistry.; PCV2.; Pig.; PMWS.
Settore VET/05 - Malattie Infettive degli Animali Domestici
2008
http://www.evsrl.it/vet.journal/archivio/archivio_pdf/2009/4181.pdf
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/143698
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